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Additional file 1: of Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures

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posted on 11.06.2018 by Daniel Lammel, Gabriel Barth, Otso Ovaskainen, Leonardo Cruz, Josileia Zanatta, Masahiro Ryo, Emanuel de Souza, Fábio Pedrosa
Theoretical full diagram and rationally. Figure S1A. Overall theoretical diagram of expected interactions in the pH range between 4 and 6 with the microbial community structure. Boxes represent soil variables and the arrows the interactions (soluble Al+ 3 is toxic for plant roots and some bacteria; Fe, Mn, Cu, Zn, and B are nutrients that in high concentrations may be toxic; and P, K, Ca, Mg, and NO3 are nutrients rarely toxic to plants). In this study, the gradient was produced by liming application, with expected direct effect on pH, and Ca and Mg values. The indirect effects are the “spillover” effect of the pH in the other soil and plant variables. Indirect effect 1 is mainly related to the solubility of elements, while indirect effect 2 is related to these effects on plant growth and nutrient cycling. SOM is the abbreviated form for soil organic matter, CEC for cation exchange capacity, and WC for water content. Temperature and WC are considered in this diagram only for the survey day of greenhouse fluxes (proxy for microbial activity). Figure S1B. Effect of pH in the relative availability of important ions related to soil fertility (Fe, Cu, Mn, Zn, Al, Mo, Cl, P, N, S, B). (PDF 526 kb)

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