Activatable Rotor for Quantifying Lysosomal Viscosity in Living Cells
journal contributionposted on 19.02.2016 by Lu Wang, Yi Xiao, Wenming Tian, Liezheng Deng
Any type of content formally published in an academic journal, usually following a peer-review process.
We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.