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Activatable Rotor for Quantifying Lysosomal Viscosity in Living Cells

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journal contribution
posted on 19.02.2016 by Lu Wang, Yi Xiao, Wenming Tian, Liezheng Deng
We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.