ppat.1007198.g006.tif (1.02 MB)
hg-UNC-25 synthesizes GABA in H. glycines.
figure
posted on 2018-08-16, 17:57 authored by Ziduan Han, Sita Thapa, Ursula Reuter-Carlson, Hannah Reed, Michael Gates, Kris N. Lambert, Nathan E. SchroederThe hg-unc-25 cDNA from H. glycines partially rescues the C. elegans unc-25(e156) mutant. In wild-type C. elegans, GABAergic neurons are detected in the head and ventral nerve cord (arrowheads) using anti-GABA staining. In the C. elegans unc-25(e156) mutant, GABA is not produced and GABAergic neurons cannot be detected using anti-GABA staining. A plasmid construct containing hg-unc-25 cDNA driven by the C. elegans unc-25 promotor partially rescues the C. elegans unc-25(e156) mutant as seen by GABA-immunoreactivity (arrowheads). Arrows indicate the expression of coel::RPF used as a co-injection marker. Scale bars, 10 μm.
History
Usage metrics
Categories
Keywords
heterologous rescuemuscle atrophyventral nerve cordimmobile stagesbody wall muscle atrophycell-specific muscle atrophymotor neuronssex-specific renewalgene expressionplant-parasitic nematodesphylogenetically diverged plant-parasitic nematode Meloidogyne incognitaMale Hmobilityplant-parasitic nematode Hglycines GABA synthesis-encoding gene hg-unc -25hg-unc -25nematode Heterodera glycinesmuscle renewaltransmission electron microscopyGABA-immunoreactive neuronsplant-parasitic nematode developmentadult males
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC