supplymentary_figure1.pdf

Supplemental Figure 1.

Relationship between body and muscle mass. This figure shows the relationship between body mass and the mass of three muscles (gastrocnemius, soleus, quadriceps) in various experimental groups. Panels A-C represent the Non-DM-CTRL and DM-CTRL groups, while panels D-F show the DM-CTRL, DM-DEX, and DM-Dex/Tofo groups.

Methodology:

Male KK-Ay/Ta Jcl (KK-Ay) and KK/Ta Jcl (KK) mice (12 weeks old) were purchased from CLEA Japan (Tokyo, Japan). Mice were housed in pathogen-free conditions with free access to a pelleted diet (CE‐2, CLEA Japan) and water. Mice were randomly assigned to four groups: Non-DM-CTRL, DM-CTRL, DM-Dex, and DM-Dex/Tofo. The DM-Dex/Tofo group received 0.015% tofogliflozin in their diet for 2 weeks. At 14 weeks, grip strength and endurance were measured. Mice were euthanized, and their muscles (gastrocnemius, soleus, quadriceps) were collected, dissected, and weighed.

Ethics Approval:

All procedures adhered to the guidelines of Juntendo University and were approved by the Ethics Review Committee for Animal Experimentation (Approval No.: 2024096).

Results:

A significant positive correlation between body mass and the mass of all three muscles was observed across all groups, suggesting that body mass influences muscle mass in both diabetic and treated conditions.