siRNAs which alter SW480-membrane-associated E-cadherin.
(A) Summary of the outcome of the secondary validation screen. The number of active siRNA for E-cadherin negative and positive regulators are indicated. At least 2 out of 4 siRNA duplexes must statistically recapitulate the primary SMARTpool screen to be considered a hit. (B) Representative images from the deconvoluted screen of αE-cadherin and Hoechst staining, 72 hours post transfection of positive regulators with selected duplex siRNAs (25nM) for mock, CTNNBIP1, CTNND1, DOCK3, DUSP4 and ITGB4. Scale bar; 50 μM, 20x magnification. (C and D) Functional categorization of candidate E-cadherin positive (C) and negative (D) regulatory genes identified from the screen (Panther Classification GO-Slim Molecular Functions, System Version 15.0).