pone.0240746.g004.tif (5.91 MB)

siRNAs which alter SW480-membrane-associated E-cadherin.

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posted on 15.10.2020, 17:44 by Lauren E. King, Hui-Hua Zhang, Cathryn M. Gould, Daniel W. Thomas, Lachlan W. Whitehead, Kaylene J. Simpson, Antony W. Burgess, Maree C. Faux

(A) Summary of the outcome of the secondary validation screen. The number of active siRNA for E-cadherin negative and positive regulators are indicated. At least 2 out of 4 siRNA duplexes must statistically recapitulate the primary SMARTpool screen to be considered a hit. (B) Representative images from the deconvoluted screen of αE-cadherin and Hoechst staining, 72 hours post transfection of positive regulators with selected duplex siRNAs (25nM) for mock, CTNNBIP1, CTNND1, DOCK3, DUSP4 and ITGB4. Scale bar; 50 μM, 20x magnification. (C and D) Functional categorization of candidate E-cadherin positive (C) and negative (D) regulatory genes identified from the screen (Panther Classification GO-Slim Molecular Functions, System Version 15.0).