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qPCR verification of concentration- and depot-dependent effects of glucocorticoids on selected, known glucocorticoid target genes.

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posted on 22.12.2016 by R. Taylor Pickering, Mi-Jeong Lee, Kalypso Karastergiou, Adam Gower, Susan K. Fried

(A) PCK1, (B) LPL, (C) GILZ, and (D) IL-6. Data are mean ± SEM, n = 5–7 independent subjects. The X-axis is a log scale. Significant depot differences at each [Dex] are indicated by an asterisk (*, p < 0.05, paired t-test of log transformed values). Repeated measures ANOVA verified a significant Dex effect in both depots for each gene (Dex effect, p ≤ 0.002). All doses in both Om and Abdsc were significantly different from 0 nM Dex (p ≤ 0.05, Dunnett’s test).