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qPCR of 40 DNA extracts for the vertebrate mitochondrial 16S rRNA gene.
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posted on 2021-09-30, 17:36 authored by Kristy I. Azzopardi, Myra Hardy, Ciara Baker, Rhian Bonnici, Stacey Llewellyn, James S. McCarthy, Rebecca J. Traub, Andrew C. SteerStool DNA with Cqs over 25 (mean+3SD, dotted line) underwent repeat DNA extraction. Mean with standard deviation derived using duplicate qPCRs.
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vertebrate mitochondrial genespecies level (<negative results dueinternal amplification controlsecond multiplex reactionpositive stools determineddna extraction controlsybr green qpcrremove potassium dichromatediv >< pmain human sthtrichuris trichiura strongyloides stercoralis necator americanus ascaris lumbricoides release sth dnastool samples collectedpotassium dichromatehuman stooltrichiura stercoralis lumbricoides americanus multiplex assays40 stoolsn duodenale ceylanicum ancylostoma transmitted helminthstested usingstaged systemsingle cyclescalable systemqpcr dataqpcr ).prevent falsepcr inhibitorshookworms );global populationbased kitambient temperature40 seconds15 weeks
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