p62 is decreased in C9orf72 knockout mouse brains.
A) Schematic representation of C9orf72 mouse tissue analysis. C9orf72-/- or wild-type mice were fed normal chow or amino acid-deficient chow (low- protein diet) for 4 weeks prior to harvesting tissue. B) Immunohistochemistry analysis of p62 in mouse brain. Brain of mouse on low protein diets were harvested and stained with p62 antibodies. p62 is decreased in C9orf72-/- brains compared to wild type brain, although it is expressed in the same region of brain. No p62 aggregates detected. C) Immunoblot analysis of brain homogenates from C9orf72 KO and wild-type animals fed control or low protein diet. C9orf72 KO mice show a decrease in p62 levels compared with wild-type littermates under the low protein diet condition. D) Quantification of p62 levels in brain homogenates derived from C9orf72 KO and wild-type animals fed control or low-protein diet. C9orf72 KO mice show a slight but significant decrease in p62 levels when compared with wild-type littermates under the low protein diet condition (n = 3, *p<0.05). Student’s t test is used and data is presented as mean ± SEM.