mTORC1 activation in podocytes resulted in progressive glomerulosclerosis.
(A) Tsc2Δpodocyte developed progressive glomerulosclerosis between 4 and 8 weeks of age. Renal tissues from Tsc2Δpodocyte and control mice at 4, 6, and 8 weeks of age were stained with periodic acid-Schiff (PAS). Representative glomeruli from Tsc2Δpodocyte and control mice are shown. Various degrees of glomerulosclerosis, partial glomerulosclerosis and protein casts in tubules (asterisks), and glomerulosclerosis with synechia formation (arrowhead) are shown. Scale bar: 50 μm. (B) Transmission electron microscopy (TEM) shows partial flattening and disorganization of podocyte foot processes (arrows). Scale bar: 5 μm. (B, bottom) Glomerular basement membrane thickness in Tsc2Δpodocyte at 4 weeks of age was comparable with that in age-matched control mice (within arrowheads). Scale bar: 1 μm.