mTORC1 activation in podocytes causes proteinuria and increased mortality.
(A) Kaplan-Meier survival plots for Tsc2Δpodocyte and control mice. A significant increase in mortality was found in Tsc2Δpodocyte compared with control mice. Nphs2-Cre (black line), n = 9; Tsc2flox/flox (blue line), n = 10; Tsc2Δpodocyte (red line), n = 32. (B) At 3 weeks, Tsc2Δpodocyte became albuminuric. SDS-PAGE gel shows a microliter of urine was loaded for each lane. (C) Seven-week follow-up of Tsc2Δpodocyte for proteinuria (n = 10 per group). Results are expressed as means ± s.d. White bar, Nphs2-Cre; dotted bar, Tsc2flox/flox; black bar, Tsc2Δpodocyte. Results are expressed as means ± s.d. *P < 0.05 compared with age-matched controls.