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iPSC-based therapy downregulated the expression of proinflammatory genes in the lungs of MCT-induced PAH rats.

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posted on 2016-02-03, 22:01 authored by Wei-Chun Huang, Meng-Wei Ke, Chin-Chang Cheng, Shih-Hwa Chiou, Shue-Ren Wann, Chih-Wen Shu, Kuan-Rau Chiou, Ching-Jiunn Tseng, Hung-Wei Pan, Guang-Yuan Mar, Chun-Peng Liu

Semi-quantitative RT-PCR (qRT-PCR) was used to detect the expression of several proinflammatory genes including IL-1β, IL-6, IL-12α, IL-12β, IL-23 and INFγ. RNA extracted from the lung tissue of the MCT-induced PAH rats and controls was reverse transcribed for semi-quantitative RT-PCR. The data were normalized according to 36b4 mRNA levels and represented as a value relative to the PBS control group. The results are shown as the means ± SD for 5–8 rats per group. *p<0.05 vs. PBS group; p<0.05 vs. MCT group.