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iFAST yields antibiotic resistance profile of pathogenic bacteria in 1 to 3 h.

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posted on 30.05.2019, 17:23 by Mehmet F. Cansizoglu, Yusuf Talha Tamer, Michael Farid, Andrew Y. Koh, Erdal Toprak

Bacterial cultures with initial OD of approximately 10−6 were grown in different concentrations of antibiotics, and their densities were periodically monitored in RUSD. Heat map plots show growth of bacteria as a function of time and antibiotic dosage. Colored bars indicate ODs of bacterial cultures. Red diamonds indicate MIC values measured at different time points at which bacterial growth is statistically significant (OD > LOQ). Below each heat map plot, a dose-response curve is provided for the first statistically significant MIC measurement. A calibration curve is generated for every strain before every measurement, and LOQ values are shown by using dashed magenta lines. (a) Time-resolved dose-response measurements for laboratory strains of P. aeruginosa (PAO1), E. coli (MG1655), and S. aureus (RN4220) in levofloxacin, a DNA gyrase inhibitor. (b) Time-resolved dose-response measurements for a clinical E. coli isolate (ET-CI28) in levofloxacin, P-T (a beta-lactam cell-wall synthesis inhibitor and beta-lactamase inhibitor), and amikacin (an aminoglycoside). (The data for Fig 3 can be found in S2 Data). iFAST, in Fiber Antibiotics Susceptibility Testing; LOQ, limit of quantification; MIC, minimum inhibitory concentration; OD, optical density; P-T, piperacillin-tazobactam; RUSD, rapid ultrasensitivity detector.

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