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Download fileiELISA-GFP validation.
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posted on 2021-11-22, 18:27 authored by Carlos Chacón-Díaz, Ana Zabalza-Baranguá, Beatriz San Román, José-María Blasco, Maite Iriarte, Dariana Salas-Alfaro, Gabriela Hernández-Mora, Elías Barquero-Calvo, Caterina Guzmán-Verri, Esteban Chaves-Olarte, María-Jesús Grilló, Edgardo MorenoThe sera of the 25 S19-GFP vaccinated heifers (Table 1) were taken at 16 weeks after vaccination and used as positive controls. The negative control sera (n = 143) were taken from brucellosis-free unvaccinated controls (n = 118) and sera from all experimental heifers before vaccination (n = 25) and 118 Brucella infected bovines. The dotted arrow indicates the iELISA-GFP cut-off resulting in 100% diagnostic sensitivity and specificity was estimated as OD = 0.198.
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widely distributed zoonosisrose bengal testgreen fluorescent proteindiv >< p5 × 10serologically positive animalstagged vaccine allowsparental s19 straingfp antibody responsebrucella abortus identifying vaccinated animalss19 induces antibodieslps antibodies preceded9 abortus s19 vaccinelps antibodiesimmunized animalsgfp antibodiesb gfp vaccinevaccinated withs19vaccinated cattlevaccinated boviness19 gfpboosted animalswestern blottingthree yearssustained vaccinationsmooth lipopolysaccharideseveral weeksseveral countriesserological identificationprotective efficaciespotential advantagesmultiplex pcrlive attenuatedindirect elisafive heifersfield strainsconjunctival routescompetitive elisachromosome ii
