siRNA targeting NRF1 mRNA ablates SPAST promoter function.
A) Cartoon showing the structure of the pGL3e-SPAST-promoter-luciferase vector, and the inhibitory mechanisms of siRNA action. Symbols are as for Fig. 1A. B) The plasmid pGL3e-SPAST-promoter was co-transfected into SK-N-SH cells with pSUPER shRNA vectors that target either NRF1, luciferase, or negative control (Arl2). *, P<0.05.