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sAC is present in the pancreatic acinar cell.

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posted on 2013-02-20, 02:04 authored by Thomas R. Kolodecik, Christine A. Shugrue, Edwin C. Thrower, Lonny R. Levin, Jochen Buck, Fred S. Gorelick

(A) RNA was isolated from pancreatic acini and the testis and PCR was performed using primers, which can distinguish full length (sAC-fl) from truncated sAC (sAC-t). Duplicates of cDNA from two different preparations of acini are shown (B) Immunoblot for sAC using sAC Ab (R21). Lanes: T:testis, L:liver and P:pancreas A:acini (50 µg protein/lane). (C) Diagram adapted from Wuttke et.al. [51] and Farrell et.al. [14] of sAC isoforms including full length (sACfl), truncated (sACt), somatic (sACsom) and a putative somatic truncation (putative sACsomtic-t). Also indicated are the PCR primer sites (P), the site of the 53 nt deletion(Δ), the site of Ab R21 recognition (R21) and the two catalytic domains (C1 and C2).

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