sAC is present in the pancreatic acinar cell.
(A) RNA was isolated from pancreatic acini and the testis and PCR was performed using primers, which can distinguish full length (sAC-fl) from truncated sAC (sAC-t). Duplicates of cDNA from two different preparations of acini are shown (B) Immunoblot for sAC using sAC Ab (R21). Lanes: T:testis, L:liver and P:pancreas A:acini (50 µg protein/lane). (C) Diagram adapted from Wuttke et.al.  and Farrell et.al.  of sAC isoforms including full length (sACfl), truncated (sACt), somatic (sACsom) and a putative somatic truncation (putative sACsomtic-t). Also indicated are the PCR primer sites (P), the site of the 53 nt deletion(Δ), the site of Ab R21 recognition (R21) and the two catalytic domains (C1 and C2).