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rqRT-PCR analysis of the AMP genes diptericin, metchnikowin, and defensin in mbn2 cells.

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posted on 24.05.2012, 01:06 authored by Kavita Bitra, Richard J. Suderman, Michael R. Strand

Cells were transfected with pIZT/V5-His empty vector (Control), pIZT/Ank-H4 or pIZT/Ank-5 and then immune challenged with commercial LPS as described in Figure 4. Total RNA was then isolated from cells for each treatment at 0, 2, 8 and 24 h post-immune challenge. The 0 h Control sample was standardized to a value of 1. Transcript abundance for the other time points were then expressed relative to the 0 h Control. Each treatment and time point was measured three times using independently transfected samples. Error bars indicate ± SE. Different letters above a given bar indicates that transcript abundance significantly differs.

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