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qPCR verification of differentially expressed PhoB-dependent genes identified by microarray analysis.

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posted on 2013-01-15, 02:10 authored by Shin Wakimoto, Haruyuki Nakayama-Imaohji, Minoru Ichimura, Hidetoshi Morita, Hideki Hirakawa, Tetsuya Hayashi, Koji Yasutomo, Tomomi Kuwahara

The transcriptional levels of representative genes in wild type (closed column) and phoB deletion mutant (open column) under Pi limitation (0.0066 mM of KH2PO4) is shown in relation to those in wild type under Pi rich condition (6.6 mM of KH2PO4). Annotation of each gene is as listed in Table 2: BF1576 (PhoB), BF1575 (PhoR), BF2185 (PstC), BF1828 (UpbY, product of the first gene product in PS B), BF2585 (UpbY, product of the first gene product in PS E), BF3377 (DnaJ), BF1205 (endopeptidase Clp), BF3395 (GroEL), BF3396 (GroES), BF2645 (polyphoaphate kinase), BF1576 (alkaline phosphatase), and BF2091 (cation efflux system protein). **Significantly different from the wild type strain, *Significantly different from the wild type strain (p<0.05).