qPCR verification of differentially expressed PhoB-dependent genes identified by microarray analysis.

The transcriptional levels of representative genes in wild type (closed column) and phoB deletion mutant (open column) under Pi limitation (0.0066 mM of KH₂PO₄) is shown in relation to those in wild type under Pi rich condition (6.6 mM of KH₂PO₄). Annotation of each gene is as listed in Table 2: BF1576 (PhoB), BF1575 (PhoR), BF2185 (PstC), BF1828 (UpbY, product of the first gene product in PS B), BF2585 (UpbY, product of the first gene product in PS E), BF3377 (DnaJ), BF1205 (endopeptidase Clp), BF3395 (GroEL), BF3396 (GroES), BF2645 (polyphoaphate kinase), BF1576 (alkaline phosphatase), and BF2091 (cation efflux system protein). **Significantly different from the wild type strain, *Significantly different from the wild type strain (p<0.05).