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prx-5/6 mutations likely cause a decrease in mmBCFA degradation.

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posted on 27.09.2013, 01:53 by Rencheng Wang, Marina Kniazeva, Min Han

(A) qRT-PCR data showing mRNA levels of the elo-6 gene in worms of indicated genotypes. elo-5(lf); Ex[elo-6] is a transgenic strain that significantly overexpressed the elo-6 gene and was not sufficient to suppress the L1 arrest phenotype of elo-5(lf). The data suggest that a higher level of elo-6 expression is not likely the cause of the suppression. (B) qRT-PCR data showing mRNA levels corresponding to several other FA elongation enzymes in worms of indicated genotypes. Since the elo-5 mRNA being measured is from the elo-5(gk208, deletion) allele, the significant decrease in elo-5 mRNA level is likely due to mutation-induced mRNA degradation. (C) Comparison of mmBCFA composition in the indicated strains. Percentage of C15ISO and C17ISO has a statistically significant increase in the maoc-1(lf) mutant.

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