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p115 and Sec16A associate with TRAF3 following cytosolic RNA and DNA sensor activation.

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posted on 05.07.2012, 01:25 authored by Wendy J. van Zuylen, Priscilla Doyon, Jean-François Clément, Kashif Aziz Khan, Lisa M. D'Ambrosio, Florence Dô, Myriam St-Amant-Verret, Tasheen Wissanji, Gregory Emery, Anne-Claude Gingras, Sylvain Meloche, Marc J. Servant

(A) Whole-cell lysates (HeLa cells) were prepared and subjected to immunoprecipitation assays using TRAF3 (H-20) or isotype control antibodies followed by immunoblotting for the presence of p115, Sec16A, and TRAF3. (B) HeLa cells were treated as indicated for different periods of time. Whole-cell lysates were prepared and subjected to immunoprecipitation assays using TRAF3 (H-20) antibody followed by immunoblotting for the presence of p115, Sec16A, TBK1 and TRAF3. The Native-PAGE assay was conducted on the same cellular extracts to demonstrate the dimerization and activation of IRF-3 upon indicated treatments. One of three independent experiments with similar results is shown.

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