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miR-375 overexpression downregulates GSK3.

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posted on 13.04.2015, 06:11 by Gili Nathan, Sharon Kredo-Russo, Tamar Geiger, Ayelet Lenz, Haggai Kaspi, Eran Hornstein, Shimon Efrat

A. Proteomic profiling of sorted GFP+ BCD cells infected at passages 4–6 with miR-375 or empty viral vectors. Proteins changed >2-fold are shown. p≤0.05 (n = 3 donors, each analyzed in duplicates). B-D. Immunoblotting of phospohorylated GSK-3α and GSK-3β inactive or active forms in expanded islet cells at the indicated passages. Values are mean±SE (n = 3 donors). *p≤0.05; **p≤0.01. E. Immunoblotting of total GSK-3α and GSK-3β proteins in expanded islet cells infected at passages 3–5 with miR-375 or empty viral vectors. Values are mean±SE (for GSK-3α, n = 3 donors; for GSK-3β, n = 5 donors). F. Immunoblotting of active forms of GSK-3α and GSK-3β. Values are mean±SE (n = 6 donors). G. Immunoblotting of the inactive form of GSK-3β. Values are mean±SE (n = 4 donors). H. Immunoblotting of inactive form of GSK-3α in expanded islet cells infected at passages 3–4 with miR-375 or empty viral vectors. Values are mean±SE (n = 6 donors).

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