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miR-23a and miR-let-7c regulate PDG-mediated inhibition of IL-6 expression.

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posted on 2013-10-15, 03:06 authored by Manish Garg, Julie A. Potter, Vikki M. Abrahams

TLR6- trophoblast cells were transfected with an either: an anti-miR scramble sequence; a specific anti-miR-23a inhibitor; a specific anti-let-7c inhibitor; or both the inhibitors of miR-23a and let-7c. Post-transfection, cells were treated with either no treatment (NT) or PDG (80μg/ml) for 12h, after which IL-6 mRNA levels were measured by qRT-PCR. Treatment of scramble control cells with PDG significantly reduced IL-6 mRNA levels, and these responses were significantly reversed by the presence of both the anti-miR-23a and anti-let-7c inhibitor. *p<0.05 relative to the NT control unless otherwise indicated (n=5).

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