miR-217 is downregulated in human osteosarcoma cell lines and tissues.

(A) Relative expression of miR-217 in four human osteosarcoma cell lines (MG-63, U2OS, SOSP-9607, and SAOS-2) and one normal bone cell line (hFOB) was determined by qRT-PCR. Quantification of miR-217 was measured by qRT-PCR with specific primers for miR-217 and snRNA U6. (B) Relative expression of miR-217 in 60 primary osteosarcoma tissues compared with their pair-matched nontumor tissues. Data areshown as log10 of relative ratio change of osteosarcoma tissues relative tonormal tissues. (C) Relative miR-217expressionlevels in osteosarcoma tissues and adjacent normal regions; (D)The expression of miR-217 in the osteosarcoma tissues from the patients with metastases was lower than that in non-metastases tissues. All data uses t test and is shown as mean ±SEM.