mTOR controls GRP78 synthesis in human cholangiocarcinoma cells.

<p>(A) After treated with rapamycin (Rap, 20 nM) for indicated time periods, GRP78 in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (B) After transfected with simTOR for 60 h, GRP78 in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without rapamycin (Rap, 20 nM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells. (D) After treated with tunicamycin (Tun, 2.0 µg/ml) for 24 h with or without rapamycin (Rap, 20 nM) preincubation for 1 h, GRP78 in HepG2 cells was analyzed using western blot.</p>