lncRNA-GAS5 affects the cell cycle and apoptosis of HSVSMCs.
As Negative regulatory factors of Cyclin Dependent Kinase, p27-kip1and p21-cip1 can delay the process of cell cycle acting as antagonist to Cyclin. Therefore, they were chosen to reflected cell cycle changes indirectly. A: The p27-kip1and p21-cip1 mRNA expression levels were measured by Q-RT-PCR with knockdown of lncRNA-GAS5 in HSVSMCs. Values are mean±SE, N = 3; *, P<0.05. B: The p27-kip1and p21-cip1 mRNA expression level were measured by Q-RT-PCR with overexpression of lncRNA-GAS5 in HSVSMCs. Values are mean±SE, N = 3; *, P<0.05. C: The apoptosis of HSVSMCs were measured by Flow Cytometry using Annexin V-FITC apoptosis kit. The result shows that the HSVSMCs in early apoptosis decreased with overexpression of lncRNA-GAS5. N = 3; *, P<0.05.