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igf2b is upregulated during zebrafish heart regeneration.

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posted on 2013-06-26, 08:23 authored by Ying Huang, Michael R. Harrison, Arthela Osorio, Jieun Kim, Aaron Baugh, Cunming Duan, Henry M. Sucov, Ching-Ling Lien

In situ hybridization (ISH) was performed on sham operated hearts (A) and 3 dpa (B), 7 dpa (C), 10 dpa (D) and 14 dpa (E) regenerating hearts. There is no detectable igf2b expression in sham-operated hearts by ISH, whereas strong expression is induced in endocardium (arrows in B and inset B’) and epicardium (arrows in C and inset C’) near the wound following amputation of the ventricular apex. Red dashed lines mark the amputation site and wound area. v: ventricle, ia: injured area, epi: epicardium. Scale bar = 100 µm. (F) Quantitative RT-PCR of igf 2b expression at 3, 7, 10 and 14 dpa compared to uncut hearts.