taz-/- mutant ventricle phenotype is rescued when injected with PDZ containing Taz mRNA.

(A) Embryos were injected with the indicated mRNA at the single cell stage and were fixed at 24hpf. Ventricles were visualized using atoh1a ventricle size of embryos within each group were measured, averaged, and compared amongst each other via ANOVA analysis. A one-way ANOVA revealed that there was a statistically significant difference the ventricle size between three treatment groups (F(3, 29) = [22.05], p < 0.00001). Tukey’s HSD Test for multiple comparisons found that the mean value of ventricle sizes was significantly different between taz^+/+ wild-type + 125 pg eGFP mRNA (M= 241.01 µm3 SD= 36.67) versus taz^-/- mutant + 125 pg eGFP mRNA (M= 146.50 µm3 SD= 21.00 p = 0.00001). Tukey’s HSD Test for multiple comparisons found that the mean value of ventricle sizes was significantly different between taz-/- mutant + eGFP mRNA (M= 146.50 µm3 SD= 21.00) versus taz-/- mutant + 100pg taz+/+ wild-type mRNA (M =188.02 µm3 SD = 41.26, p = 0.031). (B) Gene diagrams of Taz mRNA constructs, including full length taz, and various truncated taz mRNAs. (C) Embryos were injected with the indicated mRNA at the single cell stage and were fixed at 24hpf. Ventricles were visualized using atoh1a ventricle size of embryos within each group were measured, averaged, and compared amongst each other via ANOVA analysis. A one-way ANOVA revealed that there was a statistically significant difference the ventricle size between five treatment groups (F(4, 55) = [33.33], p < 0.00001). Tukey’s HSD Test for multiple comparisons found that the mean value of ventricle sizes was significantly different between taz^-/- mutants injected with eGFP (M = 146.50 µm3 SD = 21.00) and Taz ΔWW (M = 75.14 µm3 SD = 30.29 p = 0.00005), e GFP and ΔPDZ (M = 217.77 µm3 SD = 37.96 p = 0.00005), but no significant differences between eGFP injected compared to Taz ΔCC (M 120.33 µm3 SD 33.55 p = 0.36) or Taz ΔTA (M 141.20 µm3 SD 39.81 p = 1.00). Representative images of embryos with ventricle visualized with atoh1a in situ of embryos at 24 hpf. Scale bars = 100 µM.2023.

(TIF)