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nlrp3 was required for STSLS caused by S. suis.

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posted on 06.06.2019, 17:55 by Lan Lin, Lei Xu, Weihua Lv, Li Han, Yaozu Xiang, Lei Fu, Meilin Jin, Rui Zhou, Huanchun Chen, Anding Zhang

The nlrp3-deficient mice (nlrp3-/-) and its wild-type mice (nlrp3+/+) were infected with S. suis epidemic strain SC-19. (A) Cytokine levels in peritoneal lavage fluids at 6 h post-infection were determined using ELISA kits (two-tailed, unpaired t-tests, n = 4). (B) The bacterial load in the blood at 6 h post-infection was determined (two-tailed, unpaired t-tests, n = 4). (C) Blood levels of AST, ALT, LDH and CK at 6 h post-infection (two-tailed, unpaired t-tests, n = 4). (D) H&E staining of infected tissue sections from nlrp3-/- or nlrp3+/+ mice at 6 h post-infection with S. suis epidemic strain SC-19. Congestion in the lung and spleen is indicated by a “red arrow”, infiltration of inflammatory cells in the lung is indicated by a “hollow arrow”, vacuolated degeneration in the liver is indicated by a “black arrow”, and necrosis in the liver is indicated by a “yellow arrow”. (E) Clinical symptom scores of mice infected with S. suis epidemic strain SC-19 (two-way RM ANOVA, n = 6). (F) Survival of mice infected with S. suis epidemic strain SC-19 (log-rank test, n = 6). Error bars represented the mean ± standard deviations.

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