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ju1279 and ju993 are novel alleles of dhc-1 and dnc-4, respectively.

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posted on 21.06.2017, 17:25 by Naina Kurup, Dong Yan, Karina Kono, Yishi Jin

(A) Gene structure of dhc-1, with ju1279 and reference alleles or195 and js319 marked. ju1279 alters the N-terminal Heavy chain domain 1, or195 alters the coiled coil stalk and js319 alters region D6 of the motor. (B) Representative images of DD synapses along the DNC (Pflp-13-SNB-1::GFP (juIs137)) in adult animals. Ex-DHC-1 denotes extrachromosomal copies of wild type DHC-1. Scale bar: 10 μm. (C) Quantification of synaptic puncta in the DNC of adult animals. Data are mean ± SEM; n>10 animals per genotype. Statistics: One-Way ANOVA followed by Tukey’s posttest; ***p<0.001, ns- not significant. (D) Gene structure of dnc-4, with ju933 and the reference allele or633 also marked. (E) Quantification of synaptic puncta in the DNC (Punc-25-SNB-1::GFP (juIs1)) of adult animals. Ex-DNC-4(+) denotes extrachromosomal copies of wild type DNC-4. Data are mean ± SEM; n>8 animals per genotype. Statistics: One-Way ANOVA followed by Tukey’s posttest; ***p<0.001, ns- not significant. (F) Quantification of synaptic puncta in the DNC (Pflp-13-SNB-1::GFP (juIs137)) of adult animals. Animals were cultured at two different temperatures, the permissive (20°C) and restrictive (25°C) temperatures for or633, starting from late L1. Data are mean ± SEM; n>8 animals per genotype. Statistics: One-Way ANOVA followed by Tukey’s posttest; ns- not significant.

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