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dhc-1(ju1279) enhances anterograde transport during synapse remodeling.

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posted on 21.06.2017, 17:25 by Naina Kurup, Dong Yan, Karina Kono, Yishi Jin

(A) Schematic of imaging region (black box) in the DD neuron. SVs move in both the anterograde (blue solid arrow) and retrograde directions (pink dotted arrow) during remodeling. (B, C) Quantification of: (B) number of mobile vesicles, (C) their direction of movement during remodeling for various genotypes. Data are mean ± SEM; n = no. of animals (shown on (B)). Statistics: One-way ANOVA followed by Tukey’s posttest;*p<0.05, ***p<0.001, **p<0.01, n.s.-not significant (D Model of bidirectional cargo transport during DD neuron synapse remodeling. a) In wild type animals, kinesin (red) and the dynein (blue)-dynactin (yellow) complex transport SVs in both the anterograde and retrograde directions, with more SVs moving towards the dorsal neurite (anterograde). b) unc-116(ju972) (black stars) modifies the MT binding domain of kinesin to enhance both anterograde and retrograde SV transport. c) Almost all SVs move in the anterograde direction in dhc-1(ju1279) (green star) and dnc-4(ju993) (red star) animals, possibly due to a disruption in the interaction between the dynein-dynactin complex and SVs.

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