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bmm inhibition in the whole-body affects starvation resistance in both sexes.

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posted on 14.04.2021, 16:23 by Lianna W. Wat, Charlotte Chao, Rachael Bartlett, Justin L. Buchanan, Jason W. Millington, Hui Ju Chih, Zahid S. Chowdhury, Puja Biswas, Vivian Huang, Leah J. Shin, Lin Chuan Wang, Marie-Pierre L. Gauthier, Maria C. Barone, Kristi L. Montooth, Michael A. Welte, Elizabeth J. Rideout

(A) Median survival post-starvation was significantly higher in 5-day-old virgin Oregon-R females than in virgin Oregon-R males (p = 2 × 10−16; Log-rank test with Bonferroni correction for multiple comparison; n > 156). (B) Median survival post-starvation was significantly higher in 5-day-old virgin CMW wild-caught females than in virgin CMW males (p = 6 × 10−10; Log-rank test with Bonferroni correction for multiple comparison; n > 118). (C, D) Median survival post-starvation was significantly higher in 5-day-old virgin females than males in two isofemale strains (Mel c2.2: p = 4.4 × 10−11; Log-rank test with Bonferroni correction for multiple comparison; n > 55 and Mel c2.3: p = 1.4 × 10−13; Log-rank test with Bonferroni correction for multiple comparison; n > 110). (E, F) Median survival post-starvation was significantly higher in virgin males (E) and females (F), with ubiquitous overexpression of UAS-bmm-RNAi compared with control males (da>+ and +>UAS-bmm-RNAi) (p = 2 × 10−16 and 2 × 10−16 respectively; Log-rank test with Bonferroni correction for multiple comparison; n > 223) and females (p = 2 × 10−16 and 1.2 × 10−13 respectively; Log-rank test with Bonferroni correction for multiple comparisons; n > 176). (G, H) Median survival post-starvation was significantly higher in virgin males (G) and significantly lower in females (H) with ubiquitous overexpression of UAS-bmm-RNAi#2 (BDSC #25926) compared to control males (da>+ and +>UAS-bmm-RNAi#2) (p = 7.7 × 10−11 and 2 × 10−16, respectively; Log-rank test with Bonferroni correction for multiple comparison; n > 97) and control females (p = 9.6 × 10−9 and 4.9 × 10−5, respectively; Log-rank test with Bonferroni correction for multiple comparisons; n > 91). The p-values are listed in the following order: difference between the GAL4/UAS genotype and the GAL4 control/difference between the GAL4/UAS genotype and the UAS control. Asterisks indicate a significant difference between two sexes, two genotypes, or two time points (*p < 0.05, **p < 0.01, ***p < 0.001). Shaded areas represent the 95% confidence interval. See S1 Table for a list of all multiple comparisons and p-values; quantitative measurements underlying all graphs are available in S4 Data. bmm, brummer; BDSC, Bloomington Drosophila Stock Center; CMW, Country Mill Winery; da, daughterless; F, female; M, male; ns, no significant difference between two sexes, two genotypes, or time points; UAS, upstream activation sequence.

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