Tribolium pupal development illustrates how the adult central body becomes distinct from the larval form.
Displayed are subprojections of an anti-GFP staining of the same brain per time point, to display the development and positioning of cell clusters (i) belonging to the DM1-4 lineage and their tracts (ii) (DM1 green, DM2 light blue, DM3 dark blue, DM4 orange) and final projections into the developing central complex neuropils (FB iii, EB iv). (A-C) Fascicle switching becomes immediately prominently visible at 0% and shows a columnar division of the FB and increases in later stages. (D-F) In later pupal stages, decussated projections go into the NO, and a column divided EB. The following events are particularly highlighted: fascicle switching of DM1-3 was visible from 0% onwards (Aii, Bii, Cii), with a resulting formation of 4 columns of the FB and EB per hemisphere (earliest visible in Aiii and Eiv, marked by asterisks). EB, ellipsoid body; FB, fan-shaped body; GFP, green fluorescent protein; NO, noduli; pr, primordium. Scale bars represent 25 μm. https://figshare.com/articles/Fig7-9/11412777.