Tet1-dependent chromocenter clustering in the DNA hypomethylated ESCs.

(A): Representative immunostaining images of the surface-spread nuclei of the wild-type, Dnmt1-KO and Dnmt1/Tet1-DKO ESCs. Scale bar, 5 μm. (B): Boxplot showing the number of distinct chromocenters in the nuclei of ESCs, related to (A). In boxplots, the enter lines show the medians; box limits indicate the 25th and 75th percentiles, and whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Actual values are indicated by circles. Wild-type, n = 55; Dnmt1-KO, n = 64; Dnmt1/Tet1-DKO, n = 64. P values were calculated using the Mann-Whitney U-test. ***P < 0.001.