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T. gondii infection changes the metabolome.

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posted on 2020-04-07, 17:28 authored by William J. Olson, Bruno Martorelli Di Genova, Gina Gallego-Lopez, Anthony R. Dawson, David Stevenson, Daniel Amador-Noguez, Laura J. Knoll

Heatmap shows metabolite abundance over 48 hours of T. gondii infection. Triplicate infected and uninfected dishes of HFFs were metabolically quenched and metabolites were extracted at 8 times points (1.5, 3, 6, 9, 12, 24, 36, and 48 HPI). Metabolomes were quantified using HPLC-MS and metabolites were identified with known standards. Infected sample abundances were averaged and normalized to the average uninfected abundance then log base 2 transformed (Log2(Infected Abundance/Uninfected Abundance)) with blue being less abundant and yellow more abundant. Values represented in this heat map are listed in S3 Table.