S. Tm amiA amiC mutant strain displays chains in the inflamed gut.
Streptomycin-treated C57BL/6 mice (n = 4 mice per group) were infected by gavage (5×107 CFU) for 3 days with GFP-expressing S. Tm ssaV or ssaV amiA amiC mutants. Fecal pellets were collected, and then resuspended gently with PBS. The resulting S. Tm cells were observed by fluorescence microscopy. (A) Representative fluorescence microscopy images of GFP-expressing S. Tm in the feces at 3 dpi (400x). Scale bar, 20 μm. (B) Quantitative analyses of the experiments. Data are shown as means ± standard deviations. ns, not significant; **P < 0.01; ***P < 0.001; unpaired Student’s t-test.