ppat.1007391.g003.tif (1.93 MB)
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S. Tm amiA amiC mutant strain displays chains in the inflamed gut.

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posted on 31.10.2018, 18:35 by Mayuka Fujimoto, Ryosuke Goto, Riku Hirota, Masahiro Ito, Takeshi Haneda, Nobuhiko Okada, Tsuyoshi Miki

Streptomycin-treated C57BL/6 mice (n = 4 mice per group) were infected by gavage (5×107 CFU) for 3 days with GFP-expressing S. Tm ssaV or ssaV amiA amiC mutants. Fecal pellets were collected, and then resuspended gently with PBS. The resulting S. Tm cells were observed by fluorescence microscopy. (A) Representative fluorescence microscopy images of GFP-expressing S. Tm in the feces at 3 dpi (400x). Scale bar, 20 μm. (B) Quantitative analyses of the experiments. Data are shown as means ± standard deviations. ns, not significant; **P < 0.01; ***P < 0.001; unpaired Student’s t-test.