OsPUB16 expression pattern and the subcellular localization of its protein.

(A-C)OsPUB16 expression in response to MV (methyl viologen) (A), ABA (B) and MeJA (C) at indicated time. Relative transcription levels of OsPUB16 in the leaves of 2-week-old wild-type (Nipponbare) plants treated with 10 μM MV, 100 μM ABA or 100 μM MeJA for different time. Error bars indicate SD (n = 3 biological replicates). OsActin1 was used as the internal control. The significant difference between mock and treatment was determined by Student’s t test. **p < 0.01, ***p < 0.001. (D) Subcellular localization of OsPUB16 in the epidermal cells of Nicotiana benthamiana leaves. GFP fluorescence signals were visualized using the confocal microscope (Leica SP8). HY5-RFP, the nuclear-localized marker. Scale bars, 40 μm.