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L. pneumophila growth on mucin supplemented media.

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posted on 04.05.2020, 17:39 by Saima Rehman, Lubov S. Grigoryeva, Katherine H. Richardson, Paula Corsini, Richard C. White, Rosie Shaw, Theo J. Portlock, Benjamin Dorgan, Zeinab S. Zanjani, Arianna Fornili, Nicholas P. Cianciotto, James A. Garnett

WT and chiA mutant bacteria were grown from a starting OD660 of 0.3 in chemically defined medium in the presence of porcine mucin II at the indicated concentrations. At 0 h, 8 h and 24 h, bacterial numbers were determined by plating for CFU. N = 3. Representative graph shown above as mean and standard deviation of technical replicates in triplicate. Two other experiments showed the same trends, with no significant difference between mutant or mucin effect.