L. pneumophila growth on mucin supplemented media.
WT and chiA mutant bacteria were grown from a starting OD660 of 0.3 in chemically defined medium in the presence of porcine mucin II at the indicated concentrations. At 0 h, 8 h and 24 h, bacterial numbers were determined by plating for CFU. N = 3. Representative graph shown above as mean and standard deviation of technical replicates in triplicate. Two other experiments showed the same trends, with no significant difference between mutant or mucin effect.