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In vitro schistosomula assays (step 1).

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posted on 24.06.2021, 17:42 by Erik Koehne, Nina Zander, Miriam Rodi, Jana Held, Wolfgang Hoffmann, Rella Zoleko-Manego, Michael Ramharter, Ghyslain Mombo-Ngoma, Peter G. Kremsner, Andrea Kreidenweiss

Dot plot of individual IC50 values of 12 drugs and mefloquine control is shown. Each compound was tested in 3 independent, in vitro schistosomula experiments (except for MQ, N = 9) and individual IC50s (filled circles) are shown. Viability of schistosomula after 7 days drug exposure was assessed by microscopy. For four drugs (atovaquone, cycloguanil, doxycycline, clindamycin) the IC50 could not be calculated as no inhibition was observed. The IC50 is given in μM. Bar and lines indicate median IC50 and IQR. Dotted line: Threshold for drug activity is an IC50 < 10 μM. IQR: Interquartile range.