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In vitro recycling of 2-Cys Prx by PDI-M.

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posted on 2017-03-31, 18:10 authored by Benjamin Selles, Flavien Zannini, Jérémy Couturier, Jean-Pierre Jacquot, Nicolas Rouhier

The peroxidase activity of A. thaliana 2-Cys Prx was assessed using a coupled assay measuring NADPH oxidation. Results obtained for PDI-M() or PDI-M(a) are represented as percentages relative to the activity obtained with PDI-M fixed at 100%. Measurements were made in triplicate and error bars indicate standard deviation.