Fig 8.tif (159.34 kB)
In vitro recycling of 2-Cys Prx by PDI-M.
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posted on 2017-03-31, 18:10 authored by Benjamin Selles, Flavien Zannini, Jérémy Couturier, Jean-Pierre Jacquot, Nicolas RouhierThe peroxidase activity of A. thaliana 2-Cys Prx was assessed using a coupled assay measuring NADPH oxidation. Results obtained for PDI-M(a°) or PDI-M(a) are represented as percentages relative to the activity obtained with PDI-M fixed at 100%. Measurements were made in triplicate and error bars indicate standard deviation.
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Fe 2 S 2reduction reactions-170 mVmoduleAtypical protein disulfide isomerasesNADPH thioredoxin reductasepH 7.0.oxidationWCKHCPDI-L 1A Protein disulfide isomerasesnon-physiological substrate NADP-MDHb domains influencemulti-modular redox catalystspoplar PDI isoformsdithiol-disulfide exchange reactionsPDI-L 1aEscherichia coli expressionPDI-APDI-MNADP malate dehydrogenase
