pntd.0009074.g004.tif (1.22 MB)

In vitro RhATG5 cleavage assays.

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posted on 2021-01-29, 18:47 authored by Yanan Wang, Houshuang Zhang, Li Luo, Yongzhi Zhou, Jie Cao, Xuenan Xuan, Hiroshi Suzuki, Jinlin Zhou

(A) SDS-PAGE showing that recombinant RhATG5-His is cleaved by μ-calpain and is not cleaved by calpain in the absence of Ca2+. (B) The RhATG5 cleavage site. (C) The mutant recombinant RhATG5191-199Δ-His is not cleaved by μ-calpain. Please see S2 Data for the original images used in Fig 4.