In vitro RhATG5 cleavage assays.
(A) SDS-PAGE showing that recombinant RhATG5-His is cleaved by μ-calpain and is not cleaved by calpain in the absence of Ca2+. (B) The RhATG5 cleavage site. (C) The mutant recombinant RhATG5191-199Δ-His is not cleaved by μ-calpain. Please see S2 Data for the original images used in Fig 4.