In silico characterization of MAP7D3.
Protein BLAST of MAP7 and MAP7D3 identified two conserved regions located near the N- (− − −) and C- (……) termini of the proteins with 65 and 59% similarity, respectively. SMART identified coiled coil (CC) regions and MAP7 domain in both the proteins and a region of repeat sequences in MAP7D3 only. Net charges on different regions and their pIs were determined by EMBOSS Pepstats. Secondary structure prediction for MAP7D3 was done using SYMPRED.