Ex vivo expansion of periosteal tissue is not linear with total applied strain.

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posted on 2022-12-30, 18:48 authored by Mary M. Walker, Molly E. Baumann, John H. Alexander, Britani N. Blackstone, Christopher B. Morgan, Thomas J. Scharschmidt, Heather M. Powell

A) Photographs of periosteum at day 0 and after 10 days of culture. Note the decrease in periosteal strip width in the 10% group. B) Quantification of periosteal area normalized to day 0.

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histologic examination revealed explore culture methodology div >< p current project explores provide increased area facilitate graft incorporation total tissue size new tissue mineralization strain increased area viability via mtt mechanical strain induces human cancellous allografts floating samples serving ex vivo autogenous bone grafts mechanical expanded periosteum periosteal groups outperformed observed among groups fresh periosteum group fresh periosteum demonstrating per day strained vivo per day mechanical strain tissue area values among poor incorporation periosteal allograft daily strain porcine periosteum tissue anatomy significant increase patient ’osteogenic properties osteogenic potential natural limitation mechanical expansion ki67 staining implanted subcutaneously histological assessment highest levels freshly harvested common sequalae bone interface athymic mice alkaline phosphatase

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Ex vivo expansion of periosteal tissue is not linear with total applied strain.

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