pgen.1010131.g006.tif (1.61 MB)
BmPMFBP1 is essential for eupyrene sperm migration to female reproductive tracts.
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posted on 2022-03-21, 17:39 authored by Dehong Yang, Jun Xu, Kai Chen, Yujia Liu, Xu Yang, Linmeng Tang, Xingyu Luo, Zulian Liu, Muwang Li, James R. Walters, Yongping Huang(A) Morphologies of internal structure of ejaculatory seminalis of unmated WT and ΔBmPMFBP1 males. (B, C) The smear of the ejaculatory seminalis (ES). (D) Morphologies and internal structure of bursa copulatrix in females mated with WT and ΔBmPMFBP1 males. (E, F) The smear of the bursa copulatrix (BC) (G) Morphologies and internal structure of spermatheca in females mated with WT and ΔBmPMFBP1 males. The green arrows indicate the spermatheca. (H, I) The smear of the spermatheca (Sp). The yellow arrows indicated the eupyrene sperm/sperm bundles, the red arrows indicated the apyrene sperm. Paraffin-embedded sections were stained with hematoxylin, eosin, and Hoechst 33258.
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molecular genetics researchmodel lepidopteran insectdiv >< pdistinct genes underliebioinformatic analyses suggest“ pmfbp1 ”silkworm moth ),bombyx mori eupyrene sperm bundlespmfbp1 mori eupyrene spermlethal bmpmfbp1 b wildtype femaleswide rangetransgenic crisprthus playsubsequently generatedsperm deliverspecifically expressedsilkworm versionshared namesexual reproductionresults indicaterefseq databaseparticular focusoutstanding significanceobvious abnormalitiesmutants usingmutants rescuedmutant malesmutant femalesmale complementinfertility treatmentsinfertility phenotypeimportant focusgenbank ’evolved independentlyeupyrene spermatogenesisejaculatory seminalisdisordered insidedeficiency blockedcause infertilitycas9 systembroadly interestingacephalic spermatozoa>&# 916
