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elt-2 and the p38 pathway co-regulate immune protection.

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posted on 27.05.2015, 03:44 by Dena H. S. Block, Kwame Twumasi-Boateng, Hae Sung Kang, Jolie A. Carlisle, Alexandru Hanganu, Ty Yu-Jen Lai, Michael Shapira

A. Overlaps between sek-1 (or pmk-1) targets (Troemel et al., 2006), and elt-2 targets; gene numbers are shown. B, Survival curves for wildtype and sek-1 animals fed with designated RNAi’s during early adulthood, followed by P. aeruginosa infection; averages ± SDs for three plates (N = 92–151 per group,*p<0.0001 (Logrank test)); shown is a representative of several experiments with similar results. C, PF55G11.2::gfp worms fed with RNAi as designated during adulthood and exposed to P. aeruginosa (PA, 4 hours, N = 10–19 per group) or E. coli (EC, N = 19–25); signal quantification shown on the right, *p<3xE-6, ttest. Shown is a representative experiment of two with similar results. D-F, Gene expression in wildtype or pmk-1(km25) loss-of-function animals fed with designated RNAi’s during larval development. Shown are averages and SDs for two independent experiments D,F, basal expression (values and statistics relative to values in wt;EV (set to 1 and therefore not shown). E, Induction following 12 hours of P. aeruginosa infection, relative to basal expression in similarly-treated worms grown on E. coli. *p<0.01, **p<0.0005 (paired t-test); asterisks mark significant differences for individual gene(s), or for each of the genes in a group designated by a line.