eIF2α/ATF4 induces GRP78 accumulation in human CCA cells.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
(A) Western blot analysis of GRP78 in human cholangiocarcinoma cells. DMSO- and tunicamycin (Tun, 2.0 µg/ml)-treated HepG2 cells were used as negative and positive control, respectively. (B) RT-PCR analysis of spliced XBP1 mRNA in human CCA cells. Tunicamycin (Tun, 2.0 µg/ml)-treated QBC939 cells were used as positive control. (C) Western blot analysis of phosphorylated eIF2α and ATF4 in human CCA cells. Salubrinal (Sal, 25 µM)-treated HepG2 cells were used as positive control. (D) After transfected with ATF4 siRNA for 60 h, QBC939, RBE and HCCC-9810 cells were subjected to western blot analysis.