dUTPase silencing results in cell death and DNA strand breaks in larval imaginal discs.
figure
posted on 2012-06-07, 02:02 authored by Villő Muha, András Horváth, Angéla Békési, Mária Pukáncsik, Barbara Hodoscsek, Gábor Merényi, Gergely Róna, Júlia Batki, István Kiss, Ferenc Jankovics, Péter Vilmos, Miklós Erdélyi, Beáta G. Vértessy(A) Imaginal discs were isolated from wild type and dUTPase silenced wandering 3rd larvae and stained for TUNEL assay (shown as red dots). Discs from silenced animals showed highly increased TUNEL staining. (B) TUNEL positive cell counts in imaginal discs from wild type and dUTPase silenced wandering 3rd larvae. Error bars represent the standard error of mean. (C) Imaginal discs from wild type and dUTPase silenced 3rd wandering larvae stained against phospho-H2Av foci (white dots, some of these are appointed by white arrowheads). dUTPase depleted discs showed several nuclei with phospho-H2Av foci indicating DNA damage. Scale bar represents 50 µm.
