<i>cuf2</i>Δ and <i>meu5</i>Δ gene disruptions have opposite effects on common meiosis-specific genes.

<p><i>A</i>, Venn diagram representing the overlap between the <i>cuf2⁺</i>- and the <i>meu5⁺</i>-dependent genes. <i>B</i>, Total RNA from both the <i>pat1-114/pat1-114</i> (<i>cuf2⁺</i>/<i>cuf2⁺</i>) and <i>pat1-114</i>/<i>pat1-114 cuf2Δ/cuf2Δ</i> disruption strains was analyzed throughout meiosis and sporulation. A representative RNase protection experiment of the effect of the absence of Cuf2 on the expression of the <i>meu5⁺</i> transcript (especially 10 to 14 h after meiotic induction) is shown. <i>C–D</i>, Cultures of <i>pat1-114/pat1-114</i>, <i>pat1-114</i>/<i>pat1-114 cuf2Δ/cuf2Δ</i>, <i>pat1-114</i>/<i>pat1-114 meu5Δ/meu5Δ</i> and <i>pat1-114</i>/<i>pat1-114 cuf2Δ/cuf2Δ meu5Δ/meu5Δ</i> diploid cells were synchronously induced into meiosis under basal conditions. Total RNA was isolated from culture aliquots taken at the indicated time points. After RNA preparation, the <i>wtf13⁺</i> and <i>SPAC1B2.03c⁺</i> steady-state mRNA levels were analyzed by RNase protection assays using actin (<i>act1</i>⁺) as an internal control. The results shown are representative of three independent experiments.</p>