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() RT-PCR with RNA prepared from wild type HeLa cells (lanes 1,3 and 5) or RecQL1-siRNA HeLa cells (lanes 2, 4 and 6)

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posted on 2011-12-31, 00:28 authored by Gary LeRoy, Robert Carroll, Saw Kyin, Masayuki Seki, Michael D. Cole

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Taken from "Identification of RecQL1 as a Holliday junction processing enzyme in human cell lines"

Nucleic Acids Research 2005;33(19):6251-6257.

Published online 31 Oct 2005

PMCID:PMC1275589.

© The Author 2005. Published by Oxford University Press. All rights reserved

Primer pairs for RecQL1 were used for reactions loaded in lanes 1 and 2, RAD54L for lanes 3 and 4, and RAD51C for lanes 5 and 6. Products were separated by agarose gel electrophoresis and stained with ethidium bromide. () Table summarizing spontaneous and MMC induced SCE in wild type and RecQL1-siRNA HeLa cells. The mean number of SCEs per cell ± the standard deviation and the number of cells counted are shown. () Differentially stained chromosome spreads from wild type HeLa cells (left panel) and RecQL1-siRNA HeLa cells (right panel). This experiment was repeated 3 times with similar results.

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