Yeast One hybrid Assay.
figure
posted on 2013-11-14, 03:54 authored by Antonio Palazzo, Simona Marconi, Valeria Specchia, Maria Pia Bozzetti, Zoltán Ivics, Ruggiero Caizzi, René Massimiliano MarsanoLeft column: LacZ reporter system activation in yeast by full-length transposase expressed due to the interaction with the entire 5′TIR (A), the outer DR (three repeats) (C), the middle DR (three repeats) (E) or the inner DR (three repeats) (G). Right column: Bari1 transposase lacking the DNA binding domain completely fails to interact with the Bari1 5′ TIR or with its DRs (B, D, F, H).
