YUC1 overexpressing embryo sacs show extra egg cells and synergid cells at abnormal positions.
A, Confocal image showing DR5::GFP activity localized inside the embryo sac of YUC1 overexpressing female gametophytes. B, GFP signal in A is overlapped with a DIC image. C, A WT embryo sac showing the expression of a specific synergid marker. D, a YUC1 overexpressing embryo sac showing specification of two extra synergid-like cells at a chalazal position (arrows). E, WT embryo sac showing the expression of an egg cell-specific marker. F, A YUC1 overexpressing embryo sac showing expression of the marker in two extra cells at a central position (arrows). Scale bar 20 μM.