Figure_5.tif (273.69 kB)
Download file

XMetS potentiates insulin-mediated 2-deoxy-D-glucose uptake in L6 muscle cells, but does not enhance the growth of cancer cells.

Download (0 kB)
figure
posted on 2014-02-12, 03:22 authored by John A. Corbin, Vinay Bhaskar, Ira D. Goldfine, Daniel H. Bedinger, Angela Lau, Kristen Michelson, Lisa M. Gross, Betty A. Maddux, Hua F. Kuan, Catarina Tran, Llewelyn Lao, Masahisa Handa, Susan R. Watson, Ajay J. Narasimha, Shirley Zhu, Raphael Levy, Lynn Webster, Sujeewa D. Wijesuriya, Naichi Liu, Xiaorong Wu, David Chemla-Vogel, Steve R. Lee, Steve Wong, Diane Wilcock, Mark L. White

A. L6 cells expressing both isoform B of the hINSR and GLUT-4 were preincubated with either XMetS or control antibody for 60 minutes at 37°C followed by a 10 minute incubation with increasing concentrations of insulin. [3H]-2-deoxy-D-glucose (2DG) was added and uptake was measured after 20 minutes. B. MCF-7 human breast cancer cells were incubated for 48 hours at 37°C with increasing concentrations of insulin in the presence of either XMetS or control antibody, and cell proliferation was determined by CellTiter Glo® assay. C. Saos-2 human osteosarcoma cells were incubated for 72 hours at 37°C with increasing concentrations of insulin in the presence of either XMetS or control antibody, and cell proliferation was determined by CellTiter Glo® assay. The mean ± SD from triplicate determinations are shown.

History