XMetS potentiates insulin-mediated 2-deoxy-D-glucose uptake in L6 muscle cells, but does not enhance the growth of cancer cells.

A. L6 cells expressing both isoform B of the hINSR and GLUT-4 were preincubated with either XMetS or control antibody for 60 minutes at 37°C followed by a 10 minute incubation with increasing concentrations of insulin. [³H]-2-deoxy-D-glucose (2DG) was added and uptake was measured after 20 minutes. B. MCF-7 human breast cancer cells were incubated for 48 hours at 37°C with increasing concentrations of insulin in the presence of either XMetS or control antibody, and cell proliferation was determined by CellTiter Glo® assay. C. Saos-2 human osteosarcoma cells were incubated for 72 hours at 37°C with increasing concentrations of insulin in the presence of either XMetS or control antibody, and cell proliferation was determined by CellTiter Glo® assay. The mean ± SD from triplicate determinations are shown.